首页> 外国专利> METHOD FOR PREPARING STOOL SAMPLE, SOLUTION FOR PREPARING, STOOL SAMPLE, AND KIT FOR COLLECTING STOOL

METHOD FOR PREPARING STOOL SAMPLE, SOLUTION FOR PREPARING, STOOL SAMPLE, AND KIT FOR COLLECTING STOOL

机译:制备样品的方法,制备溶液,样品和用于收集样品的工具箱

摘要

Provided in the present invention are a method for preparing a stool sample without any need for complicated operations which is capable of efficiently recovering a nucleic acid or the like originating from mammalian cells, such as the cells exfoliated from the large intestine, in the stool; a solution for preparing a stool sample and a kit for stool collection which are used in the above method; and a method for recovering and analyzing the nucleic acid in stool using the stool sample prepared by the above method. More specifically, provided are a method for preparing a stool sample which is a method for preparing a stool sample in order to efficiently recover a nucleic acid from the stool sample, the method characterized in that the collected stool is mixed with a solution for preparing a stool sample which has a water-soluble organic solvent as an active ingredient; a solution for preparing a stool sample which is used in the method; a kit for collecting stool; a method for recovering a nucleic acid characterized by recovering a nucleic acid originating from indigenous enteric bacterium and a nucleic acid originating from an organism other than indigenous enteric bacterium at the same time from the stool sample prepared by the preparation method; and a method for analyzing a nucleic acid that analyzes the nucleic acid recovered by the method for recovering a nucleic acid.
机译:本发明提供一种无需复杂操作即可制备粪便样品的方法,该方法能够在粪便中有效地回收源自哺乳动物细胞如从大肠脱落的细胞的核酸等。在上述方法中使用的制备粪便样品的溶液和用于收集粪便的试剂盒;以及使用通过上述方法制备的粪便样品回收和分析粪便中核酸的方法。更具体地,提供了一种制备粪便样品的方法,该方法是一种制备粪便样品以便从粪便样品中有效回收核酸的方法,该方法的特征在于将收集的粪便与用于制备粪便的溶液混合。以水溶性有机溶剂为有效成分的粪便样品;该方法中使用的制备粪便样品的溶液;收集粪便的工具包;一种用于回收核酸的方法,其特征在于,同时从由所述制备方法制备的粪便样品中回收源自本地肠细菌的核酸和源自本地肠细菌的生物以外的核酸;分析核酸的方法,该核酸分析通过所述核酸回收方法回收的核酸。

著录项

  • 公开/公告号IN2010DN03697A

    专利类型

  • 公开/公告日2010-11-05

    原文格式PDF

  • 申请/专利权人

    申请/专利号IN3697/DELNP/2010

  • 发明设计人 TANIGAMI YASUO;NAGAOKA TOMONORI;

    申请日2010-05-25

  • 分类号C12M1/26;C07H21;C12N15;G01N1/28;C12M1;C07H21/02;G01N33/48;C12N15/09;C12Q1/68;

  • 国家 IN

  • 入库时间 2022-08-21 18:46:00

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