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Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool

机译:粪便样品的制备方法,粪便样品的制备溶液和粪便采集试剂盒

摘要

A method for preparing a stool sample without any need for complicated operations is provided which is capable of efficiently recovering a nucleic acid originating from mammalian cells, such as the cells exfoliated from the large intestine, in the stool. A solution for preparing a stool sample and a kit for stool collection are also provided. The collected stool is mixed with a solution for preparing a stool sample which has a water-soluble organic solvent as an active ingredient. A method is disclosed for recovering a nucleic acid including recovering a nucleic acid originating from indigenous enteric bacterium and a nucleic acid originating from an organism other than indigenous enteric bacterium at the same time from the stool sample prepared by the preparation method.
机译:提供了一种无需复杂操作即可制备粪便样品的方法,该方法能够在粪便中有效地回收源自哺乳动物细胞,例如从大肠脱落的细胞的核酸。还提供了用于制备粪便样品的溶液和用于粪便收集的试剂盒。将收集的粪便与用于制备粪便样品的溶液混合,该溶液具有水溶性有机溶剂作为活性成分。公开了一种用于回收核酸的方法,该方法包括从通过该制备方法制备的粪便样品中同时回收源自本地肠细菌的核酸和源自非本地肠细菌的生物的核酸。

著录项

  • 公开/公告号US8754204B2

    专利类型

  • 公开/公告日2014-06-17

    原文格式PDF

  • 申请/专利权人 YASUO TANIGAMI;TOMONORI NAGAOKA;

    申请/专利号US20100782537

  • 发明设计人 YASUO TANIGAMI;TOMONORI NAGAOKA;

    申请日2010-05-18

  • 分类号C07H21/00;C07H21/02;

  • 国家 US

  • 入库时间 2022-08-21 16:04:33

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