A PROCESS FOR THE ESTIMATION OF BOD USING A NOVEL IMMOBILIZED MICROBIAL MEMBRANE

摘要

The present invention relates to a for the estimation of BOD using a novel immobilized microbial membrane which comprises of a synergistic mixture of the isolated bacterial namely, Aeromonas hydrophila, Pseudomonas aeruginosa, Yersinia enterocolitica, Serratia liquefaciens, Pseudomonas fluorescens, Enterobacter cloaca, Klebsielfa oxytoca, Citrobacter amalonaticus and Enterobacter sakazaki. The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for the monitoring of BOD load of synthetic sample i.e., Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.