HIGHLY SPECIFIC POLYCLONAL ANTIBODIES AND A METHOD THEREOF

摘要

Disclosed herein are highly specific, polyclonal antibodies of individual Xenopus histones H2A, H2B, H3 and H4 expressed in E. coli and their uses thereof. These four polyclonal antibodies are raised in rabbit and tested against highly purified HeLa core histones. Significantly all of these antibodies are able to identify individual members of the core-histones with high specificity. Thus these antibodies will be very useful tools in studying the in vivo status of the histones during several cellular processes.