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METHOD FOR GENERATING STABLE CELL LINES EXPRESSING HIGH LEVELS OF A PROTEIN OF INTEREST.
METHOD FOR GENERATING STABLE CELL LINES EXPRESSING HIGH LEVELS OF A PROTEIN OF INTEREST.
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机译:产生表达感兴趣蛋白高水平的稳定细胞系的方法。
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摘要
This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX.
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