Bioreactor multi camera with infusion bidirectional integrated in a culture system for application in tissue engineering strategies

摘要

This invenu00c7u00c3o refers to a bioreactor integrated in a culture system that can be used to stimulate the colonizau00c7u00c3o and accession cell expansion and differentiation of cells under conditions of culture highly controlled and monitored.The bioreactor (1) it is a multi chamber of the infusion device containing an innovative retention structures to support cell growth and cavity distribution of flow of culture medium.When integrated in the culture system described, the bioreactor can be used to increase and to direct growth and cellular differentiation, through the establishment of a dynamic environment of culture.This is capable of homogu00c9nea and simultaneously exposing various matrices celularizadas two-dimensional and / or three-dimensional cutting voltage via the infusion uni - or bidirectional culture medium. The culture system is sealed, being independent and self-sufficient as regards the establishment of its internal temperature and gas composition of the culture medium.The system also includes devices for monitoring and control of parameters of contu00cdnuos culture as well as to the quantification of several nutritional and components dissolved in the culture medium.