embryogenese wild grapes (vitis silvestris somatic calli and homogeneous mass production.

摘要

several accessions of wild grape and treatment, belonging to the vitis silvestris. and from several regions in the north of tunisia, were evaluated for in vitro regeneration ability is. in tunisia, the vines lambrusques populations are threatened with extinction due to deforestation, surpaturages and fires.it is in this context, a process of in vitro regeneration via somatic embryogenesis has been established, in order to preserve and exploit the genetic deterioration.the high multiplication is popular in various research programs, such as engineering, cryopreservation, in vitro production of "artificial seed production, as well as in studies of bio prospecting.to do this, the induction of callus from leaf and europiennes of lambrusques was carried out on the middle hit and pool (1987), enriched with 2 mg.r1 2,4-d and 2.5 mg.r1 of tdz. the callus induced by the way of solid culture have a low capacity embryogene, mainly related with the use of the gelling agent.in order to overcome this problem, a second fluid medium composed of cp (1987), enriched in mg. 1 2,4-d and 1 to 1.5 mg. tdz has been established and has led to the expression and proliferation of somatic embryos.on the other hand, the production of callus was compact, homogeneous texture, pigmented or unpigmented, from sheets of lambrusques has necessitated the use of the environment (1987), enriched in pc - 1 1 mg. 2,4-d. the callus masses of homogeneous and non embrygu00e9nu00e8s can be exploited through the cu ltures cells in liquid, in order to accumulate the production of secondary metabolites.these molecules may be a biotechnological interest.