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Improved process for the preparation of Activation of soluble conjugated polysaccharide - Protein and its purification by hidrofu00d3bica interaction
Improved process for the preparation of Activation of soluble conjugated polysaccharide - Protein and its purification by hidrofu00d3bica interaction
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机译:可溶性共轭多糖蛋白的制备及其hidrof u00d3bica相互作用纯化的改进工艺。
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摘要
The present Inventors, describe an improvement in the technical polisacaru00eddico activated with the Activation of Antigen reactive tetragluoroborato 1 - cyano - 4 - (dimethylamino) PYRIDINIUM (cdapbf4) previously reported (lees et al.1996) and purification of the polysaccharide - Protein conjugate obtained by this method directly from the Reaction mixture obtained by the above process in one step by Hydrophobic Interaction Chromatography on gels or hidroxipropiladas matrices Hidroxialquiladas,That allows the obtaining of the polysaccharide - Protein conjugate both as free polysaccharide Protein conjugate without simplifying the overall process of production and providing a product with better characteristics to be used as immunogen
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