METHOD FOR SIMULTANEOUS DETECTION OF STAPHYLOCOCCUS AUREUS AND ASSOCIATED ANTIBIOTIC RESISTANCE GENES

摘要

THE PRESENT INVENTION RELATES TO A METHOD FOR SPECIFIC DETECTION OF STAPHYLOCOCCUS AUREUS, AS WELL AS FOR SPECIFIC DETECTION OF THE ANTIBIOTIC RESISTANCE GENES THAT ARE BEING CARRIED BY THE STRAIN FOR BIOLOGICAL SPECIMENS. ACCORDING TO THE INVENTION, THE METHOD IS BASED ON DNA PRIMERS AND PROBES IN POLYMERASE CHAIN REACTION (PCR) FOR SIMULTANEOUS DETECTION OF A SPECIES-SPECIFIC GENE AND ANTIBIOTIC-RESISTANCE GENES, WHEREBY, DETECTION OF ALL AMPLIFICATION GENE PRODUCTS IS INDICATIVE OF STAPHYLOCOCCUS AUREUS HAVING MULTIPLE DRUG RESISTANCE TOWARDS METHICILLIN AND AMINOGLYCOSIDE. THE INVENTION ALSO RELATES TO A DIAGNOSTIC KIT FOR CARRYING OUT THE INVENTIVE METHOD.