SPECIFIC OLIGONUCLEOTIDES FOR THE DETECTION, IDENTIFICATION AND QUANTIFICATION OF MYCOPLASMA AGALACTIAE ISOLATED.

摘要

Specific oligonucleotides for the detection, identification and quantification of Mycoplasma agalactiae isolates.;The oligonucleotides of the present invention have a nucleotide sequence that is specific for a region of the p40 gene specific to the Mycoplasma agalactiae species. Said oligonucleotides act as primers and probe, respectively, against the target sequence of said gene.;The detection, identification and quantification of Mycoplasma agalactiae isolates is carried out by means of standard PCR technology in any of its known variants, especially through the use of real-time PCR by means of TaqMan type probes, and is characterized in that for the amplification of the region of the p40 gene of the Mycoplasma agalactiae isolate to be determined said oligonucleotides are used.