Method to quantitate receptor G protein coupled junction (GPCR) -protein g by using a cell membrane array.

摘要

Method to quantify the receptor binding G protein coupled (GPCR) -protein G by using a cell membrane array.; The method comprises (i) contacting a candidate compound not marked with a cell membrane array in the presence of labeled GTP or an analog thereof, hydrolyzable labeled, under conditions allowing interaction between said compound and said GPCR present in said cell membranes, and between said labeled GTP or analog thereof and said G protein present in said membranes; (Ii) washing; and (iii) quantifying the signal obtained by the binding of GTP (or analog) to said G protein labeled Application to the analysis of the interaction between compounds and proteins cell membrane receptors and intracellular signaling mechanisms triggering this interaction mechanism mediated by said compounds.