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METHOD OF QUANTIFYING THE G PROTEIN-COUPLED RECEPTOR (GPCR)/G PROTEIN COUPLING USING A CELL MEMBRANE ARRAY
METHOD OF QUANTIFYING THE G PROTEIN-COUPLED RECEPTOR (GPCR)/G PROTEIN COUPLING USING A CELL MEMBRANE ARRAY
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机译:使用细胞膜阵列量化G蛋白偶联受体(GPCR)/ G蛋白偶联的方法
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摘要
The invention relates to a method for quantifying G protein-coupled receptor (GPCR)-G protein binding by means of using a cell membrane array, which comprises (i) putting an unlabeled candidate compound in contact with a cell membrane array in the presence of labeled GTP or of a labeled, non-hydrolyzable analog thereof, in conditions allowing the interaction between said compound and said GPCR present in said cell membranes, and between said labeled GTP or analog thereof and said G protein present in said membranes (ii) washing and (iii) quantifying the signal obtained due to the binding of the labeled GTP (or analog) to said G protein. It is applicable in the analysis of the interaction between compounds and cell membrane receptor proteins and of the intracellular signaling mechanisms triggering this interaction mechanism mediated by said compounds.
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