IMMOBILIZATION PROCEDURE OF A DEHYDROGENASE GLUTAMATE.

摘要

Immobilization procedure of a glutamate dehydrogenase. br / br / This application describes the preparation of a derivative of a Thermus spp glutamate dehydrogenase (GDH) immobilized-stabilized by covalent multipunual binding. The soluble enzyme is already very stable from pH 6 to pH 10, even at 60 ° C, recognizes NAD, NADH, NADP, and NADPH. Its immobilization on glyoxyl supports allows the stabilization of both its multimeric structure and the rigidity of each monomer, further increasing its thermoresistance, and extending the range of use to pH below 5 (where the soluble enzyme is inactivated by dissociation). The immobilized derivative is resistant to solvents, high temperature, drastic pHs, etc. This immobilized derivative of the GDH, therefore, would have excellent possibilities of use as a cofactor regenerating enzyme in enzymatic reduction-oxidation processes or as a biosensor.