Saccharomyces cerevisiae cect 11783 yeast farm, genetically modified with pectinolitic activity: its method of obtaining and applying. (Machine-translation by Google Translate, not legally binding)

摘要

Strain of yeast saccharomyces cerevisiae cect 11783 genetically modified able to express and specify in commercially useful quantities an endopolygalacturonase, for use in the food and beverage industry. To achieve this goal, the linear dna fragment of defined sequence with the gene coding for endopolygalacturonase (pgu1) bound in transcriptional fusion to the promoter of the phosphoglycerate kinase (pgk1) gene both were included in the genome of a strain of saccharomyces cerevisiae. Of origin saccharomyces cerevisiae, devoid of bacterial sequences or of any other organism different from the one mentioned. This strain can be used to synthesize the enzyme and then purify and add it to fruit juices to improve clarification and extraction performance, or, as in fermented products, that the yeast produces the enzyme directly during the process, without needing to be added to the medium. (Machine-translation by Google Translate, not legally binding)