METHOD FOR IDENTIFYING THE DIANA PROTEIN OF AN AGENT AND METHOD FOR EXPLORING A THERAPEUTIC AGENT FOR DIABETES USING THE DIANA PROTEIN.

摘要

A method of exploring an agent for treating diabetes, comprising [1] a step of allowing (1) a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2, (2) a polypeptide comprising the sequence of amino acids represented by SEQ ID NO: 2 or an amino acid sequence of SEQ ID NO: 2 in which 1 to 10 amino acids thereof are deleted, substituted and / or inserted and which also binds to biguanide and / or inhibits the activation of AMPK by biguanide, due to overexpression, (3) a polypeptide comprising an amino acid sequence that has an identity of 90% or more with the amino acid sequence represented by SEQ ID NO: 2 and that it also binds to biguanide and / or inhibits the activation of AMPK by biguanide due to overexpression or (4) a cell transformed with a vector comprising a polynucleotide encoding the polypeptide described in (1) to (3) being in contact with a substance a in say in coexistence of biguanide and [2] a step of analyzing the binding of said polypeptide with the substance to be tested.