METHOD FOR DETERMINING THE CONTRIBUTION OF BIOMASS TO COD

摘要

The method comprises the application of molecular microbiological techniques of in situ hybridation (FISH), staining with DAPI and cell viability to determine the contribution to the chemical oxygen demand of the specific groups of bacteria present in biological waste-water purification processes (heterotrophic bacteria, autotrophic bacteria and polyphosphate-accumulating bacteria, PAO). This method permits the calibration of the mathematical methods of activated sludges in a direct manner without needing to have recourse to optimisation of the results, thereby reducing the uncertainty when determining the calibration parameters for a specific system of waste water treatment.