Biofilm forming organisms are incubated to form a biofilm on projections byproviding a flow of liquid growth medium acrossprojections, the direction of the flow of liquid being repeatedly changed, andan assay made of the resulting biofilm. Biofilm formingorganisms are incubated to form a biofilm on projections arranged in rows,with several projections in each row, while providing a flow ofliquid growth medium across each row of projections, and an assay made of theresulting biofilm. Sensitivity of the biofilm to antimicrobialreagent may be determined by treating the projections with antimicrobialreagent before carrying out the assay, by treating each row ofprojections with a different antimicrobial reagent, and each of theprojections in a row with a different concentration of antibacterial reagent.A biofilm assay device includes a vessel including at least one channel forflow of liquid growth medium, projections arranged in at leastone row and having a support for supporting the projections within thechannel, and a tilt table to flow liquid growth medium along eachchannel in different directions across the projections. A further biofilmassay device includes a vessel including channels for flow of liquidgrowth medium, projections arranged in rows and having a support forsupporting the projections within the channels, and a rocking tableto rock the vessel and flow liquid growth medium along each channel across theprojections.
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