METHODS OF PURIFYING RECOMBINANT HUMAN ERYTHROPOIETIN FROM CELL CULTURE SUPERNATANTS

摘要

The present invention relates, in general, to a method of purifyingrecombinant human erythropoietin (EPO). The present invention alsorelates to a substantially pure EPO. The method comprises a differentialprecipitation, an hydrophobic interaction chromatography, variousconcentration and diafiltration steps, tandem anionic and cationic exchangechromatographies and molecular exclusion chromatography forthe obtaining of pure EPO. The method does not comprise high performanceliquid chromatography steps. The invention also comprisesthe EPO obtained according to the claimed procedure.