DETECTION OF NO BY USING GUANYLYL CYCLASE AND CGMP PRODUCTION AS A READOUT SYSTEM

摘要

A method for measuring nitric oxide synthase activity, characterized in themethod comprises; (a) providing a solution comprising a nitric oxide synthaseand a suitable substrate under conditions which allow synthesis of nitricoxide; (b) adding guanylyl cyclase and GTP under conditions which allowsynthesis of cGMP; (c) measuring cGMP.