PROBES FOR DETECTING PROTEIN NUCLEAR TRANSPORT AND METHOD FOR DETECTING AND QUANTIFYING PROTEIN NUCLEAR TRANSPORT USING THE SAME

摘要

A convenient and highly accurate method for detecting protein nuclearmigration due to an endogenous or foreign substance in a local area such as avital cell or an individual organism with the use of a pair of probes fordetecting protein nuclear migration, characterized in that the probescomprising a probe I having the protein, the nuclear migration of which is tobe detected and quantified, connected to either the N-terminal side or the C-terminal side of a fused structure [intein-C/labeled protein-C] wherein atleast a C-terminal side polypeptide obtained by halving intein is connected toa C-terminal side polypeptide obtained by halving a labeled protein in thisorder, and another probe II having a nuclear localization signal connected toeither the N-terminal side or the C-terminal side of a fused structure[labeled protein-N/intein-N] wherein at least the remaining N-terminal sidepolypeptide obtained by halving intein is connected to the remaining N-terminal side polypeptide obtained by halving the labeled protein in thisorder.