METHODS FOR SCREENING INITIAL CODONS PROVIDING DESIRED EXPRESSION LEVELS OF PROTEINS, AND METHODS FOR TUNING EXPRESSION AND PRODUCTION OF RECOMBINANT PROTEINS

摘要

Disclosed herein is a method for screening an initial codon providing a desired expression level of a protein by changing randomly the initial codon of the gene, introducing the gene into a cell to isolate an individual gene and performing a colony polymerase chain reaction (PCR) to amplify the gene, and expressing the gene to a protein using the cell-free protein synthesis system; and a method for controlling the expression of the recombinant protein; and a method for preparing the recombinant protein by using same.