Methods for screening initial codons providing desired expression levels of proteins, and methods for tuning expression and production of recombinant proteins

摘要

The present invention initial codon of the gene (initial codon), ie more than one codon +2 to +13 , in particular after changing the +2 and +3 after a random codon by introducing into these cells separated into individual genes and amplified by colony PCR, using a cell-free protein synthesis system by expressing each of the gene , the protein , the objective how to dig the initial codon provided by the protein expression level , and relates to a method for controlling expression of the recombinant protein and the production method using the same, in the protein production can be a systematic and precise adjusting means , the active protein to the desired level It can be expressed at a high speed .