METHOD AND KIT FOR EXAMINATION OF CELLS USING N-(9-ACRIDINYL)MALEIMIDE (NAM) OR USING 7-DIETHYLAMINO-3-((4'-(IODOACETYL)AMINO)PHENYL)-4-METHYLCOUMARIN (CPI)

摘要

The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells. The present invention relates to the use of N-(9-acridinyl)maleimide (NAM) or to the use of 7-diethylamino-3- ((4'-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species (e.g., sulphur-containing species) present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead) cells. The present invention also relates to the use of NAM or to the use of 7-diethylamino-3-((4'- (iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of NAM or with the use of 7-diethylamino-3-((4'-(iodoacetyl)amino)phenyl)-4- methylcoumarin (CPI). The invention further relates to compositions used in methods for analyzing cells.