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OPTIMIZING SELECTION OF SRM TRANSITIONS FOR ANALYSIS OF BIOMOLECULES BY TANDEM MASS SPECTROMETRY
OPTIMIZING SELECTION OF SRM TRANSITIONS FOR ANALYSIS OF BIOMOLECULES BY TANDEM MASS SPECTROMETRY
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机译:串联质谱法优化生物分子分析的SRM转变选择
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摘要
Methods for selecting a set of SRM transitions for a peptide of interest include selecting a first transition based on sensitivity criteria and selecting at least a second transition based on selectivity criteria. A determination of the uniqueness of the first transition combined with the at least a second transition is made. When the combination of the first transition and the at least a second transition is determined to be unique to the peptide of interest, a sample containing the peptide of interest is subjected to a SRM workflow by monitoring the first transition and the second transition. Also described is an apparatus for carrying out the methods.
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