MARKER REAGENTS WITH A PYRIDINE RING WITH A DIAZOMETHYL FUNCTION SUBSTITUENT METHODS FOR SYNTHESIS OF SAID REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES

摘要

The invention relates to marker reagents of respective formulae (C) or (D), where R1 is in particular a detectable marker, • R2, R3 are, independently, H, NO2 Cl, Br, F, I, OR, SR, NR2, NHCOR, COOR where R = alkyl or aryl, • R4 is H,alkyl or aryl, L is a bridging arm. The invention further relates to a method for synthesising a marker reagent, a method for marking a biological molecule, a marked biological molecule obtained by said method, a method for marking and fragmenting a single or double strand nucleic acid, a marked nucleic acid obtained by said method, a detection kit for a target nucleic acid comprising a marked nucleic acid, a solid support to which a reagent is fixed and a method for trapping nucleic acids. The invention is preferably applied to diagnostics.