METHOD AND SYSTEM FOR WIDE-FIELD MULTI-PHOTON MICROSCOPY HAVING A CONFOCAL EXCITATION PLANE

摘要

A wide-field microscope includes a stage (50) to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source (10) to produce a substantially parallel beam of excitation light (25) having a single photon energy less than an absorption energy required for single photon excitation of the fluorescent material.