CONTINUOUS ASSAY METHOD FOR MEASURING RECEPTOR- AND ION CHANNEL-MEDIATED CALCIUM RESPONSE AND SUBSEQUENT GENE EXPRESSION

摘要

A method of utilizing a calcium-responding photoprotein selected from the group consisting of the calcium-responding aequorin photoprotein from jellyfish, and its functional equivalents, and measuring rise in intracellular calcium; and continuously thereafter measuring, with the cells left alive within the same container, and employing the same substrate, induction and suppression of gene expression, utilizing a reporter-gene assay that uses and measures a reporter gene selected from the group consisting of calcium non-responding Renilla luciferase and its functional equivalents.