METHODS FOR IDENTIFYING DIFFERENCES IN ALTERNATIVE SPLICING BETWEEN TWO RNA SAMPLES

摘要

Provided are methods of separating one or more alternate isoform of a target gene, which alternate isoform is present in a sample population of RNAs but not in a subtractive population of RNAs. Compositions comprising a subpopulation of RNA:cDNA duplexes and a subpopulation of unhybridized RNA fragments are also provided.