A RESISTANCE-SCREENED TUMOR STEM CELL, ITS ANTIGEN COMPOSITION, AN ANTI-TUMOR DENDRITIC CELL LOADING WITH SAID ANTIGENS, THEIR PREPARATION METHODS, USES AND KITS THEREOF AS WELL AS A DENDRITIC CELL VACCINE

摘要

Resistance-screened tumor stem cells, their preparation method, use of such tumor stem cells for manufacturing anti-tumor medicaments and a kit for preparing said tumor stem cells. An antigen composition of said tumor stem cells, its preparation method, use of such antigen composition for manufacturing anti-tumor medicaments and a kit for preparing said antigen composition. Dendritic cells loading with said antigens obtained by pulsing normal dendritic cells with said antigen composition of said tumor stem cells, its preparation method, use of such dendritic cells for manufacturing anti-tumor medicaments, a kit for preparing such dendritic cells, and a vaccine of such dendritic cells. The medicaments prepared from the resistance-screened tumor stem cells, the antigen composition of such tumor stem cells and the dendritic cells loading with said antigens provided by the present invention, can induce immune response in vitro and in vivo to kill tumor stem cells which cause tumor recurrence, which make it possible to overcome tumor resistance and to radically cure tumor recurrence and metastasis.