Method for a genome wide identification of expression regulatory sequences and use of genes and molecules derived thereof for the diagnosis and therapy of metabolic and/or tumorous diseases
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Method for a genome wide identification of expression regulatory sequences and use of genes and molecules derived thereof for the diagnosis and therapy of metabolic and/or tumorous diseases
Method for a genome wide identification of expression regulatory sequences and use of genes and molecules derived thereof for the diagnosis and therapy of metabolic and/or tumorous diseases
The invention is directed to the use of particular human genes, nucleic acids hybridizing to said genes, and gene products encoded thereby in the context of the diagnosis and/or therapy of metabolic and/or cancerous diseases, preferably of diabetes mellitus and/or colorectal cancer, wherein the gene is selected from the group of the human chromosomal genes having at least one expression regulatory sequence according to matrix 1 ("de novo" HNF4α matrix) in the range of 100000 nucleotides upstream or downstream of their transcription start site in the human genome, and wherein the at least one expression regulatory sequence according to matrix 1 is located within the chromosomal position specified by particular start and end sites.;The invention further relates to a method for a genomewide identification of functional binding sites at specifically targeted DNA sequences with high resolution, wherein the method comprises, or preferably consists of, the steps of:a. chromatin immunoprecipitation andb. DNA-DNA hybridisation for thec. de novo identification of gene targets.
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机译:本发明针对特定的人类基因,与所述基因杂交的核酸以及由此编码的基因产物在代谢和/或癌性疾病,优选糖尿病和/或结肠直肠癌的诊断和/或治疗中的用途。癌症,其中该基因选自在其转录起始位点上游或下游100000个核苷酸的范围内具有至少一种根据基质1的表达调控序列的人类染色体基因(“从头”HNF4α基质)。人基因组,并且其中至少一种根据基质1的表达调节序列位于特定起始和终止位点指定的染色体位置内。本发明进一步涉及一种在全基因组范围内鉴定特异性靶向DNA上功能结合位点的方法具有高分辨率的序列,其中所述方法包括或优选由以下步骤组成:一种。染色质免疫沉淀和b。 DNA-DNA杂交C。 de novo I>鉴定基因靶标。
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