FLUORESCENT LIGHT MICROSCOPIC MEASUREMENT OF A SAMPLE USING RED-SHIFTED STOKES LINES

摘要

According to the invention, fluorescent light microscopic measurement of a sample (2) is carried out by converting a fluorescent dye in the sample (2) from one state to another state using light (8) of a given wavelength, wherein light (10) of a different wavelength is injected at such an intensity into an optic fibre (13) selected such that Raman scattering is stimulated in said optic fibre (13) to such an extent that a light spectrum emitted from the optic fibre (13) has at least one red-shifted Stokes line (19 to 28) in addition to the injected wavelength, the half peak height width of which is less than half the separation from the adjacent blue-direction line (18 to 28) of the light spectrum (17) and one wavelength is selected from one of the red-shifted Stokes lines(19 to 28) and fluorescent light (6) from the sample (2) is locally measured.