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Improved epitope displaying phage

机译:改进的表位展示噬菌体

摘要

The present invention relates to libraries of display phage which display various mutant epitopic peptides or protein domains with potential to bind to a target material of interest. Site-specific protease cleavable linkers may be incorporated into these phages to overcome the problem of irreversible binding to target materials. When the displayed insert is a mini-protein, a reagent which cleaves the crosslink may be used to facilitate release of a tightly bound display phage. The invention also relates to providing a more balanced library of epitopic peptide display phage.
机译:本发明涉及展示噬菌体的文库,该文库展示了各种突变表位肽或蛋白质结构域,它们可能与目标靶物质结合。可以将位点特异性蛋白酶可裂解的接头掺入这些噬菌体中,以克服与靶材料不可逆结合的问题。当展示的插入物是微蛋白时,裂解交联的试剂可用于促进紧密结合的展示噬菌体的释放。本发明还涉及提供更平衡的表位肽展示噬菌体文库。

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