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OVEREXPRESSION METHOD OF FOREIGN GENE FROM PLANT BODY USING 2B GENE OF CUCUMBER MOSAIC VIRUS WHICH SUPPRESSES GENE SILENCE
OVEREXPRESSION METHOD OF FOREIGN GENE FROM PLANT BODY USING 2B GENE OF CUCUMBER MOSAIC VIRUS WHICH SUPPRESSES GENE SILENCE
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机译:利用黄瓜花叶病毒2B基因抑制植物基因沉默的植物外源基因过表达方法
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摘要
PURPOSE: An overexpression method of a foreign gene in plant body using cucumber mosaic virus is provided to simply and massively produce useful protein with low price.;CONSTITUTION: An overexpression of foreign gene in plant using infectious clone of cucumber mosaic virus(CMV) comprises: a step of preparing a recombinant vector containing RNA1a gene of CMV; a step of inserting the foreign gene into an expression vector having restriction enzyme site; a step of transforming Agrobacterium tumefaciens to the recombinant vector to obtain transformant; and a step of mixing Agrobacterium tumefaciens transformant into plant body.;COPYRIGHT KIPO 2010
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