RECOMBINANT STREPTOMYCES STRAIN IN WHICH RAPAMYCIN PRODUCTIVITY IS ENHANCED THROUGH IN VIVO ACYL-COA INCREASE

摘要

PURPOSE: A method for preparing recombinant Streptomyces strain and enhancing rapamycin productivity is provided to improve rapamycin productivity and to produce antibacterial, anti-cancer, and immunity suppressing drugs.;CONSTITUTION: A recombinant Streptomyces strain in which rapamycin productivity contains foreign accA1 gene, pccB gene, and prpE gene. The recombinant Straeptomyces strain is obtained using an expression vector(Pset152-pcc) in which a gene is introduced in PermE downstream. The gene encodes carboxylase(pcc) and propionate CoA ligase(PrpE). The foreign accA1 gene and pccB gene is obtained from Streptomyces coelicolor. The foreign prpE gene is obtained from Salmonella typhimurium.;COPYRIGHT KIPO 2010