METHOD FOR PREPARING HUMAN BLOOD COAGULATION FACTOR VIII USING CELL LINE WHICH PRODUCES SYNTHETIC PROTEIN AND VON WILLIBRAND FACTOR

摘要

PURPOSE: A method for preparing human blood coagulation factor VIII is provided to increase productivity of human blood coagulation factor VIII and to highly express the human blood coagulation factor VIII even in serum-free medium.;CONSTITUTION: A method for preparing human blood coagulation factor VIII comprises: a step of transfecting stabilized von willibrand factor expression vector in a CHO(Chinese hamster ovary) cell line; a step of transfecting an expression vector of synthetic protein having linked signal amino acid(19 amino acids), heavy chain(1-740 amino acids), B domain(741-745, 1640-1648) and light chain(1649-2332) of the human blood coagulation factor VII to the transfected CHO cell line; a step of amplifying a target gene; and a step of culturing methotrexate-adapted CHO cell line in serum-free medium.;COPYRIGHT KIPO 2010