MESENCHYMAL STEM CELL DERIVED FROM FETUS IN AMNIOTIC FLUID AND A METHOD FOR MASSIVELY PRODUCING GROWTH FACTOR USING THE SAME

摘要

PURPOSE: A mesenchymal stem cell derived from fetus in amniotic fluid by culturing is provided to ensure pluripotency and to massively produce growth factors.;CONSTITUTION: A mesenchymal stem cell derived from fetus in amniotic fluid is prepared by culturing the fetus-derived cells in a medium containing 10% of FBS(fetal bovaine seru) and 1-20 ng/ml of bFGF(basic fibroblast growth factor). The medium is low glucose DMEM(Dulbecco's Modified Eagle's Medium) medium. The mesoderm-derived cell is adipocyte, osteocyte, or chondrocyte. A method for massively producing the human growth factor from mesenchymal stem cell comprises: a step of isolating fetus-derived cell from a lying-in women; a step of sub-culturing in a medium containing FBS and bFGF to obtain mesenchymal stem cells; and a step of culturing the mesenchymal stem cell in serum free medium for 2-7 days to prepare a conditioned medium.;COPYRIGHT KIPO 2011