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RICE STRIPE VIRUS RESISTANT TRANGENIC RICE PLANT AND A METHOD FOR PRODUCING THE SAME

机译:水稻条纹抗病毒转基因水稻植物及其生产方法

摘要

PURPOSE: A rice stripe virus resistant transgenic rice plant is provided to express RSV-CP-SW gene in a sense direction and anti-sense direction and to obtain dsRNA.;CONSTITUTION: A vector for transforming a plant, pANDA-RSV-CP-SW contains a coat protein gene of rice stripe virus of sequence number 1. A pathogen resistant transgenic plant is obtained by transforming with transformed Agrobacterium tumefaciens. The plant is a rice plant. A method for producing the transgenic rice plant comprises: a step of isolating the coat protein gene of rice stripe virus of sequence number 1; a step of preparing an expression vector for transformation in which the isolated gene is inserted in each sense and anti-sense direction; a step of preparing Agrobacterium tumefaciens for plant transformation with the expression vector; and a step of transforming the rice plant using transformed Agrobacterium tumefaciens.;COPYRIGHT KIPO 2011
机译:目的:提供一种水稻条纹病毒抗性转基因水稻植物,以在有义方向和反义方向上表达RSV-CP-SW基因并获得dsRNA 。;组成:用于转化植物的载体pANDA-RSV-CP- SW含有序列号为1的水稻条纹病毒的外壳蛋白基因。通过用转化的根癌农杆菌转化获得抗病原体的转基因植物。该植物是水稻。一种转基因水稻植物的生产方法,包括:分离序列号为1的水稻条纹病毒外壳蛋白基因的步骤;制备用于转化的表达载体的步骤,其中在各个有义和反义方向上插入分离的基因;用表达载体制备根癌农杆菌用于植物转化的步骤; ;以及使用转化的根癌农杆菌转化水稻的步骤。; COPYRIGHT KIPO 2011

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