Expression vectors for monitering differentiation of mesenchymal stem cell into the cardiomyogenic lineage, and monitering methods of differentiating into the cardiomyogenic lineage by using the same

摘要

A recombinant expression vector for monitoring the differentiation of stem cell to a cardiomyogenic cell is provided to detect the differentiation and use for cell therapy of cardiovascular disease and stability and efficiency of stem cell therapy. A recombinant expression vector pDsRed-Express-1/mMLC, pDsRed-Express-1/rMLC, pEGFP-1/cTn-1, or pDsRed-Express-1/cTn-1 for monitoring the differentiation to a cardiomyogenic cell comprises a promoter inducing the cardiomyogenic cell-specific expression and reporter gene which is operably linked. The promoter inducing the cardiomyogenic cell-specific expression is the promoter myosin light chain 2v promoter of the sequence number 1(SEQ ID NO:1), rat myosin light chain 2v promoter of the sequence number 2, or myocardial troponin I promoter of the sequence number 3. A method for monitoring the differentiation to cardiomyogenic cell comprises: a step of preparing a mesenchymal stem cell; a step of transducing the recombinant expression vector into a prepared stem cell; and a step of confirming the expression of reporter gene in the transduced stem cell.