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Biomarker for identification of exposure to malachite green and the method of identification using thereof
Biomarker for identification of exposure to malachite green and the method of identification using thereof
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机译:用于鉴定孔雀石绿暴露的生物标志物及其鉴定方法
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摘要
A biomarker for identifying the exposure to malachite green is provided to specifically increase or decrease the expression by the malachite green and monitor the pollution by the malachite green. A biomarker for identifying the exposure to a malachite green comprises: a gene registration number (GeneBank) AB000584(Growth differentiation factor 15); a gene registration number NM_030581(Homo sapiens WD repeat domain 59)(WDR59); a gene registration number AK026623(Homo sapiens nuclear-encoded mitochondrial cytochrome C oxidase Va subunit); and a gene registration number DQ282439(Homo sapiens isolate B2-1-06 mitochondrion(cytochrome C oxidase subunit1). A method for identifying the exposure to the malachite green comprises: a step of isolating RNA from a human somatic cells and control somatic cells; a step of synthesizing RNA to cDNA (complementary DNA); a step of hybridizing the cDNA with DNA microarray chip; a step of analyzing DNA microarray chip; and comparing the expression level between control group and sample group.
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