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Cells expressing CD40L for activation of B cells
Cells expressing CD40L for activation of B cells
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机译:表达CD40L激活B细胞的细胞
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摘要
A cell expressing CD40L ligand (CD40L) for B cell activation is provided to obtain an activated B cell whose antigen presentation and proliferation are improved and develop a drug of antivirus or anti-tumor. A cell expressing CD40L for enhancing B cell proliferation and antigen presentation is manufactured by cloning the CD40L gene from a CD4+ T cell and transducing to HLA (human leukocyte antigen) deficient animal cell. The CD40L comprises a human CD40L gene having a sequence of the sequence number 1(SEQ ID NO:1). The HLA deficient animal cell is K562(ATCC CCL 243). A method for producing the K562 cell line which expresses CD40L comprises: a step of cloning from cDNA (complementary DNA) by using restrict enzyme, EcoRI and NotI; and a step of inserting CD40L into the expression vector and transducing into the K562 cell line.
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机译:提供表达用于B细胞活化的CD40L配体(CD40L)的细胞以获得其抗原呈递和增殖得以改善并开发抗病毒或抗肿瘤药物的活化的B细胞。通过从CD4 + T细胞克隆CD40L基因并将其转导至缺乏HLA(人类白细胞抗原)的动物细胞来生产表达CD40L的细胞,以增强B细胞的增殖和抗原呈递。 CD40L包含具有序列号1(SEQ ID NO:1)的序列的人CD40L基因。 HLA缺陷动物细胞是K562(ATCC CCL 243)。生产表达CD40L的K562细胞系的方法包括:通过使用限制酶,EcoRI和NotI从cDNA(互补DNA)克隆的步骤;将CD40L插入表达载体并转导至K562细胞系的步骤。
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