METHOD OF OBTAINING OF IMMOBILIZED NANO-CELLULASES

摘要

FIELD: biotechnologies.;SUBSTANCE: food carboxymethylcellulose is dissolved in buffer solution or alkalised distilled water with pH of 9.5-10.0, and cellulose produced by culture Trichoderma reesei or culture Trichoderma viride is added to filtrate of produced solution with activity of at least 50 units/g in the form of powder in the presence of 1-1.5% solution of surfactant in process of intense mixing with speed of 4500-5000 rpm. Produced emulsion is cooled down to 5-10°C, pH of reaction mixture is reduced down to 3.0-3.5 and is left for a day in refrigerator. Precipitated particles are separated from solution, and acetate buffer is added repeatedly to remained particles with pH of 4-4.5 in process of mixing, then precipitated particles that contain cellulase are separated and dried.;EFFECT: reduced dimensions of immobilised cellulase particles, increased speed of fermentative process.;3 ex