METHOD OF PRODUCING 2- dehydro-3-EPI-20-HYDROXIECDYSONE, MINOR ECDYSTEROID OF PLANT SEEDS OF FROELICHIA FLORIDANA

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to chemistry of natural compounds, specifically to enhanced synthesis of ecdysteroids - insect molting hormones. The invention describes a single-step method for synthesis of 2-dehydro-3-epi-20-hydroxyecdysone 1 during ozonation of 20-hydroxyecdysone (2) in a pyridine solution at 0-25°C for 10-20 minutes. Ecdysteroid 1 obtained that way was extracted in crystalline form with output of 83% in terms of reacted 20-hydroxyecdysone (2). Melting point and specific angle of rotation were further determined for the 20-hydroxyecdysone. The structure of the obtained product was confirmed through a combination of 1D and 2D NMR ¹H and ¹³C spectra.;EFFECT: method ensures high output of ecdysteroid via a single-step process.;1 cl, 3 ex