EXPANSION OF THE CELL SPECTRUM FOR INCLUSION OF SUBDOMINANT EPITOPES BY VACCINATION WITH ANTIGENS DELIVERED AS PROTEIN FRAGMENTS OR PEPTIDE COCKTAILS

摘要

1. A vaccine against a chronic disease, such as a bacterial, viral or parasitic infection or cancer, comprising a peptide mixture containing adjacent overlapping peptides spanning the whole amino acid sequence of a protein that is expressed during the chronic phase of the disease. ! 2. The vaccine according to claim 1, also comprising an auxiliary agent, where the auxiliary agent is cationic. ! 3. The vaccine according to claim 1, where one or more of the peptides is chemically modified. ! 4. The vaccine according to claim 1, where each peptide is included separately in liposomes to obtain a mixture. ! 5. The vaccine according to claims 1 to 4, where the peptides have a length of 10-30 amino acids, preferably 12-20 amino acids. ! 6. The vaccine according to claim 1, where the overlap with the adjacent peptide is 6-20 amino acids, preferably 10-12 amino acids. ! 7. The vaccine according to claim 1, where the protein is selected from a bacterium, such as a virulent mycobacterium, for example Mycobacterium tuberculosis, Mycobacterium africanum or Mycobacterium bovis, Mycobacterium leprae or Chlamydia trachomatis, or a virus, such as hepatitis B or C virus, or parasite as species of Leishmania, or the malaria-causing parasite Plasmodium falciparum, or from molecules expressed in malignant tumors. ! 8. The vaccine according to claim 7, where the peptides come from a protein selected from M. tuberculosis, such as ESAT6, Ag85A, Ag85B or TB10.4, or from Chlamydia trachomatis, such as CT184, CT521, CT443, CT520, CT521, CT375 , CT583, CT603, CT610 or CT681, or from hepatitis virus, or from Plasmodium falciparuman, such as Msp1, Msp2, Msp3, Ama1, GLURP, LSA1, LSA3 or CSP. ! 9. The vaccine according to claim 1, where one or more of the peptides is chemically modified. ! 10. The vaccine according to claim 9, where one or more of the peptides is chemically modified by glycosylation, formation of a compound with lipids, labeling of PAM3Cys or dodecan