SOLID-PHASE IMMUNO-ENZYME ANALYSIS (ELISA) FOR VASCULAR ENDOTHELIUM GROWTH FACTOR (VEGF)

摘要

1. A method for detecting selective forms (VEGF110 +) of vascular endothelial growth factor (VEGF) in a biological sample, comprising the steps! (a) contacting and incubating the biological sample with a capture reagent immobilized on a solid support, wherein the capture reagent is an antibody that recognizes the same epitope as 5C3 anti-human VEGF antibody, wherein said monoclonal antibody specifically binds to residues in an amount of more than than 110, from human VEGF; ! (b) separating the biological sample from the immobilized capture reagents; ! (c) contacting the immobilized molecular complex of the capture-target reagent with a detectable antibody that binds to the VEGF domains responsible for binding to the KDR and / or FLT1 receptor, or which binds to an epitope in VEGF1-110; and! (d) measuring the level of VEGF110 + bound to capture reagents using a detection tool for a detectable antibody. ! 2. The method according to claim 1, where the biological sample is taken from a person. ! 3. The method according to claim 2, where the person is a patient with a vascular, diabetic or cancerous disease, and the measurement step (d) further comprises comparing with a standard curve to determine the level of VEGF in comparison with the level in a healthy individual. ! 4. The method according to claim 1, where the biological sample is a tumor lysate, plasma, serum or urine. ! 5. The method according to claim 1, where the capture reagent is a 5C3 monoclonal antibody. ! 6. The method according to claim 1, where the immobilized capture reagents are applied as a coating on a microtiter plate. ! 7. The method according to claim 1, where the detected antibody is directly �