STRAIN LACTOCOCCUS LACTIS SUBSPLACTIS F-119-PRODUCER OF ANTIBIOTIC COMPLEX LGS AND METHOD OF PRODUCING ANTIBIOTIC COMPLEX LGS USING SAID STRAIN

摘要

FIELD: chemistry; biochemistry.;SUBSTANCE: invention relates to biochemistry. The strain Lactococcus lactis subsp.lactis 284 (F-119) is deposited in the Collection of microorganisms of the M.V. Lomonosov Moscow State University. The strain is obtained by fusing two related strains L. lactis subsp.lactis 729 and 1605 with subsequent selection of highly active versions on selective media and culturing the producer in an optimised medium. The strain is cultured in a fermentative saline medium, containing a source of carbon, nitrogen, mineral salts and yeast autolysate. The obtained culture liquid has antibiotic activity of 6800 ME/ml. The culture liquid is dried, obtaining antibiotic complex LGS, with activity of 1000000-1200000 ME/g. Components of the antibiotic complex are separated if necessary. Ratio of antibiotics LGS-B to LGS-H in the antibiotic complex is 1:4.;EFFECT: obtained strain is capable of synthesising an antibiotic complex with high activity and a wide range of antibacterial effects, effective against gram-positive bacteria, including spore and pathogenic forms, bacilli, listeria, mycobacteria, and against gram-negative bacteria, such as coliform bacteria, salmonella, psychrophilic bacteria.;3 cl, 2 tbl, 5 ex