MEDIUM THAT DOES NOT CONTAIN PROTEINS AND SERUMS, AND METHOD OF MAMMAL CELL CULTIVATION IN SUCH MEDIUM

摘要

FIELD: medicine.;SUBSTANCE: medium for mammal cell cultivation contains a synthetic medium, 0.1 to 100 g/l of soya hydrolyzate. And the molecular weight of soya hydrolyzate makes either ≤1000 Dalton, or ≤500 Dalton, or ≤350 Dalton. The method of mammal cell cultivation involves introduction of said mammal cells into a medium that does not contain proteins and serum where said medium contains the synthetic medium, 0.1 to 100 g/l of soya hydrolyzate of the molecular weight ≤1000 Dalton. The method for protein recovery from mammal cell culture involves introduction of the cells in the medium specified above, while said cells express required protein; cultivation of the specified cells in the specified medium and expression of the specified protein, thereby preparation of the mixture of the specified cells and the specified protein in the medium; purification of the specified protein from the specified mixture.;EFFECT: higher yield of mammal cells.;14 cl, 7 tbl, 9 ex