Processing organic acid from fermentation broth in which biomass is separated and filtrate is treated with acid to obtain a mixture, comprises applying the mixture on chromatography resin, rinsing with mobile phase and obtaining fractions

摘要

Method for processing organic acid from fermentation broth, where the pH-value during the fermentation is adjusted by the addition of a base, the biomass from the fermentation broth is separated and the resulting filtrate is treated with acid to obtain a mixture, which releases organic acid and further components, comprises: (a) applying the mixture on chromatography resin that is equilibrated with a suitable mobile phase and comprises one or more interconnected columns; (b) rinsing the columns with the mobile phase; and (c) obtaining different fractions. Method for processing organic acid from fermentation broth, where the pH-value during the fermentation is adjusted by the addition of a base that binds as a salt to the resulting organic acid, the biomass from the fermentation broth is separated and the resulting filtrate is treated with acid to obtain a mixture, which releases organic acid and further components, comprises: (a) applying the mixture on a chromatography resin (a continuously operated simulated moving bed chromatography) that is equilibrated with a suitable mobile phase and comprises one or more interconnected columns; (b) rinsing the columns with the mobile phase; and (c) obtaining different fractions, in which each components of the mixture are collected separately from each other based on their different retention times.