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composition for the coating of a carrier to produce a zellage, carrier to produce a zellage and processes for the manufacture of a zellage
composition for the coating of a carrier to produce a zellage, carrier to produce a zellage and processes for the manufacture of a zellage
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机译:载体的涂层以生产软弹的组合物,载体的生产软弹和制造软弹的方法
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摘要
Disclosed are a method for manufacturing a cell sheet, comprising culturing cells on a fibrin-coated surface of a substrate until the cells reach confluency, continuing the cultivation of the cells for a sufficient time period to cause the degradation of fibrin at the bottom of the cells, and detaching the cultured cells from the substrate surface in a sheet-like form to give a cell sheet; a substrate for cell sheet preparation, a surface of which is coated with fibrin; and a composition for use in coating with fibrin a surface of a substrate for cell sheet preparation, the composition comprising fibrinogen and thrombin. The invention enables to cell sheets to be manufactured by a simple manipulation using a substrate coated with a commercial, commonly available material of which safety has been confirmed.
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