tool for the transfer and the characteristic of proteins with the pseudomonas type iii secretion system

摘要

Expression vector (EV) for a chimeric protein (CP) the N-terminal fragment of which corresponds to a fusion between the N-terminus of ExoS of Pseudomonas aeruginosa and an amino acid (aa) sequence of interest (I), with the N-terminus of ExoS being the N-terminus of CP. EV comprises (5' to 3') a promoter; sequence (II) that encodes 48-96 N-terminal aa of ExoS (or of a sequence with at least 70% identity) and sequence (III) that encodes (I), is new. Independent claims are also included for: (1) cloning vector (CV) containing, 5' to 3', a promoter and (II); (2) CP in which the N-terminus comprises the first 48-96 aa from the N-terminus of ExoS, or a sequence with at least 70% identity; (3) gene encoding the CP of (2); (4) isolated strain of P. aeruginosa transformed with EV; (5) ex vivo or in vitro injection of CP into eukaryotic cells; and (6) producing CP. ACTIVITY : Cytostatic, Antiinflammatory; Antiangiogenic; Antioxidant. No biological data given. MECHANISM OF ACTION : None given.