new sars coronavirus associated with strain and its uses

摘要

An isolated and purified strain of human coronavirus (CV) associated with severe acute respiratory syndrome (SARS), is new. Isolated and purified strain of human coronavirus (CV) associated with severe acute respiratory syndrome (SARS). Its genome, in the form of cDNA, includes a Ser codon at positions 23220-23222 of the S protein or a Gly codon at positions 25298-25300 of the ORF3 gene, and an Ala codon at positions 7918-7920 of ORF1a or a Ser codon at positions 26857-26859 of the protein M gene, where all positions are referenced to the Genbank sequence AY27419.3. Independent claims are also included for: (1) isolated or purified polynucleotide (I) from the genome of the new strain; (2) isolated or purified polynucleotide (Ia) that hybridizes to (I) under highly stringent conditions; (3) fragments of (I) or (Ia); (4) pairs of primers that can amplify part of the genome of CV, or its equivalent DNA; (5) probes that can detect the genome of CV, or part of it; (6) DNA or RNA chips or filters that contain at least one of (I), (Ia) or their fragments; (7) recombinant cloning and/or expression vector that contains a fragment of (3); (8) cDNA bank that comprises the fragments of (3); (9) cells modified by the vector of (7) or the bank of (8); (10) isolated, purified protein or peptide (II) that is encoded by (I), (Ia) or their fragments; (11) mono- or poly-clonal antibodies (Ab), or their fragments, prepared by immunization with the vector of (7), the bank of (8) or (II), and able to bind to at least one (II); (12) protein or peptide chip or filter that includes at least one (II), Ab or fragment of Ab; (13) detection reagent for a SARS-associated CV that contains the new primers, probes, chips, filters, vectors, CV strain, (I), (Ia), (II), Ab or its fragments; (14) detecting CV by amplifying, from material extracted from a sample, a fragment of ORF-N by reverse transcription PCR, using the primers of (4), then detecting any amplicon formed; and (15) kit for detection of CV comprising any of the new primers, probes, DNA/RNA chips or filters, vectors, cells, isolated CV, (I) or (Ia). ACTIVITY : Virucide. No biological data given. MECHANISM OF ACTION : Vaccine.